CELL Laura Melohn, Stephen Eichhorn, Orlando Rojas  Thursday, March 25, 2010 

313 - Cellulose recalcitrance during enzymatic hydrolysis: Its induction during isolation and its reversal using an innovative new technology

Rajai H. Atalla, rhatalla@wisc.edu, Rowan S. Atalla. Research Division, Cellulose Sciences International, Madison, Wisconsin 53704, United States

Use of cellulose as a source of glucose to be used as feedstock for biofuels is burdened by recalcitrance of cellulose when it is subjected to hydrolytic enzymes. The key barrier is the prolonged time requirement for adequate hydrolytic action and associated capital investment in equipment. Recalcitrance has been assumed to be inherent in the nature of celluloses. Our studies indicate that recalcitrance is induced during isolation of most celluloses. We have developed new technology that reduces recalcitrance and makes possible significant increases in rates of conversion of celluloses by cellulase enzymes. For bleached kraft pulps we have achieved conversions of the order of 80% in ~ 20 hours, where conversion of a control is 45%. For corn stover we have achieved conversion rates that are twice those of controls. Induction of recalcitrance will be discussed and procedures for its reversal outlined.

Thursday, March 25, 2010 08:20 AM
Deconstructing the Cell Wall Structure (08:20 AM - 11:15 AM)
Location: Hilton San Francisco Union Square
Room: Continental Parlor 1/2


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